Open AccessThe expression of the Mycobacterium aurum carotenogenesis operon is not repressed by the repressor of Mycobacterium vaccae photoinducible carotenogenesis
Author(s) -
HoussainiIraqui M.,
ClavelSérès S.,
Rastogi N.,
David H.L.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05573.x
Subject(s) - operon , microbiology and biotechnology , biology , repressor , plasmid , bacteria , mycobacterium , recombinant dna , mycobacterium leprae , dna , gene , gene expression , genetics , escherichia coli , immunology , leprosy
The photochromogenic Mycobacterium vaccae ATCC 15483 was successfully transformed using the recombinant plasmid pC1 which contained a 10.83‐kb DNA fragment representing the carotenogenesis operon from the scotochromogenic species Mycobacterium aurum A + . Experiments on the ability of transformed bacteria to synthesize carotenes in the dark or upon photoinduction revealed that transformed organisms were able to synthesize carotenes in the dark. Therefore, it was concluded that the expression of the M. aurum carotenogenesis operon in M. vaccae was not under control by the repressor of the M. vaccae photo‐inducible carotenogenesis.