Open AccessCloning of DNA from a Rhodococcus strain conferring the ability to decolorize sulfonated azo dyes
Author(s) -
Heiss Gesche S.,
Gowan Bhavna,
Dabbs Eric R.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05571.x
Subject(s) - dna , strain (injury) , mutant , rhodococcus , chemistry , clone (java method) , cloning (programming) , molecular cloning , library , biology , biochemistry , microbiology and biotechnology , gene , 16s ribosomal rna , peptide sequence , enzyme , anatomy , computer science , programming language
Azo dye are recalcitrant pollutants. Two sulfonated azo dyes, Orange II and Amino black, are effectively decolorized by certain noncardioform strains of the genus Rodococcus . A mutant of one of these strains was isolated which had lost azo‐dye decolorizing ability and the strain was used to clone DNA conferring this ability, by screening of Bc /I library constructed from DNA of a decolorizing strain. The relevant genetic information was located on a 6.3‐kb fragment of DNA.