Open AccessIdentification of the cellulose‐binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS
Author(s) -
Poole Debbie M.,
Morag Ely,
Lamed Raphael,
Bayer Edward A.,
Hazlewood Geoffrey P.,
Gilbert Harry J.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05563.x
Subject(s) - clostridium thermocellum , cellulosome , identification (biology) , protein subunit , cellulose , chemistry , biochemistry , domain (mathematical analysis) , clostridium , carbohydrate binding module , cellulase , computational biology , biology , bacteria , genetics , botany , gene , mathematical analysis , mathematics
The 3′ region of a gene designated cipB , which shows strong homology with cipA that encodes the cellulosome S L subunit of Clostridium thermocellum ATCC 27405, was isolated from a gene library of C. thermocellum strain YS. The truncated S1 protein encoded by the cipB derivative bound tightly to cellulose. The cellulose‐binding domain in this polypeptide consisted of a C‐terminal proximal 167 residue sequence which showed complete identity with residues 337–503 of mature S L from C. thermocellum strain ATCC 27405. The cellulose‐binding domain interacted with both crystalline and amorphous cellulose, but not with xylan.