Open AccessTyping of methicillin‐resistant Staphylococcus aureus with IS 256
Author(s) -
Wei MingQ.,
Udo Edet E.,
Grubb Warren B.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05562.x
Subject(s) - typing , staphylococcus aureus , gentamicin , microbiology and biotechnology , transposable element , biology , methicillin resistant staphylococcus aureus , genetics , antibiotics , bacteria , genome , gene
A simple one‐step procedure is described for specifically amplyfying and labelling insertion elemement IS 256 which is associated with the gentamicin‐resistance transposon Tn 4001 . The product has been used to probe DNA digests of methicillin‐resistant Staphylococcus aureus . The resulting restriction fragment length polymorphisms were found to be able to distinguish isolates which were indistinguishable by other typing methods. The probe also hybridised with methicillin‐resistant Staphylococcus aureus which were isolated before the emergence of gentamicin resistance, demonstrating its usefulness in typing species other than those that are gentamicin‐resistant.