Open AccessNickel uptake and its localization in a cyanobacterium
Author(s) -
Singh A.L.,
Asthana R.K.,
Srivastava S.C.,
Singh S.P.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05560.x
Subject(s) - nickel , spheroplast , bioconcentration , polyphosphate , chemistry , cell wall , phosphate , nuclear chemistry , biochemistry , environmental chemistry , bioaccumulation , organic chemistry , escherichia coli , gene
Nickel bioconcentration in different cell preparations of the cyanobacterium Nostoc muscorum was examined. A two‐ or three‐fold increase in phosphate concentration over that prescribed in growth medium (58 μM), favoured nickel accumulation restricted to a threshold limit. Intact cells showed highest nickel bioconcentration (8.41 μmol mg −1 dry wt) over spheroplasts (6.19 μmol mg −1 dry wt) or polyphosphate bodies (5.88 μmol mg −1 dry wt). Such preparations derived from similar cells indicate that the cyanobacterial cell wall could accommodate around 14–19% of the total nickel taken in by the cell with the overall nickel‐bioconcentration sequence as: intact cells > spheroplasts > polyphosphate bodies > cell wall. The data suggest that polyphosphate bodies are the main sink for nickel.