Open AccessPotassium ions and changes in bacterial DNA supercoiling under osmotic stress
Author(s) -
Meury Jean,
Kohiyama Masamichi
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05559.x
Subject(s) - dna supercoil , mutant , osmotic concentration , osmotic shock , plasmid , biophysics , dna , escherichia coli , chemistry , potassium , circular bacterial chromosome , biochemistry , biology , microbiology and biotechnology , gene , dna replication , organic chemistry
Escherichia coli transiently increases both the [ATP]/[ADP] ratio and the negative supercoiling of plasmid DNA when it is shifted to high osmolarity. Here we report that a mutant lacking all saturable K + transport systems increases the negative supercoiling of the plasmid DNA under upshock but cannot further relax DNA. The mutant dnaK 756 behaves like the K + transport mutant.