Open AccessStudies on a murine model for evaluation of virulence of Streptococcus suis capsular type 2 isolates
Author(s) -
Beaudoin M.,
Higgins R.,
Harel J.,
Gottschalk M.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05552.x
Subject(s) - streptococcus suis , virulence , microbiology and biotechnology , biology , streptococcaceae , streptococcus , virology , bacteria , gene , antibiotics , genetics
Five different parameters, time of incubation of the culture, type of culture medium, inoculum, strain of inbred mice, and age of mice, were tested using the LD 50 technique to standardize a murine model for the evaluation of the virulence of Streptococcus suis capsular type 2 isolates. A model using 28 day‐old mice belonging to CF1 strain appeard to give the best results. The inoculum size was the parameter most influencing the 50% lethal dose obtained with mice. Inoculation with 1‐ml volume of a bacterial suspension instead of 0.1 or 0.5 ml decreased the LD 50 . The standardized model was used to evaluate the virulence of some isolates of known pathogenicity for pigs. The minimum lethal dose was used in the model and it appeared that the virulence of Streptococcus suis capsular type 2 isolates can be measured from highly virulent to totally avirulent.