Open AccessA transient and rapid activation of plasma‐membrane ATPase during the initial stages of osmoregulation in the salt‐tolerant yeast Zygosaccharomyces rouxii
Author(s) -
Nishi Toshiaki,
Yagi Tadashi
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05548.x
Subject(s) - osmoregulation , atpase , cycloheximide , intracellular , glycerol , biochemistry , salt (chemistry) , osmotic shock , chemistry , membrane , yeast , biophysics , biology , enzyme , salinity , protein biosynthesis , ecology , gene
Effects of various inhibitors on the intracellular accumulation of glycerol and inorganic ions in a salt‐tolerant yeast, Zygosaccharomyces rouxii , were examined for several hours during NaCl‐induced salt stress. Cycloheximide strongly inhibited the intracellular accumulation of glycerol during salt stress but chloramphenicol did not. Rapid activation of plasma‐membrane ATPase was apparent within 5 min after the start of salt stress and after 1 h a second, slower activation occurred. ATP was maintained at a higher level during salt stress than that in its absence. Experiments with various other inhibitors demonstrated a close relationship between synthesis of glycerol, activation of plasma membrane ATPase and increases in levels of ATP. These results suggest that activation by salt stress of plasma‐membrane ATPase may trigger the synthesis of glycerol for osmoregulation.