Open AccessMaintenance of broad host range vector pKT230 in marine unicellular cyanobacteria
Author(s) -
Sode Koji,
Tatara Masahiro,
Ogawa Shigeo,
Matsunaga Tadashi
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05544.x
Subject(s) - replicon , synechococcus , kanamycin , cyanobacteria , plasmid , biology , host (biology) , vector (molecular biology) , microbiology and biotechnology , botany , bacteria , gene , ecology , genetics , recombinant dna
The host range of pKT230 in marine unicellular cyanobacteria was investigated. The broad host range vector, pKT230, was successfully transferred by transconjugation to Synechococcus sp. PCC7335, NKBG15031a and Synechocystis 7a. pKT230 was found to be maintained as an autonomous replicon in strains NKBG15031a and 7a. pKT230 stability in Synechococcus sp. NKBG15041c, in which pKT230 was found to be maintained as an autonomous replicon in our previous report [1], was also studied. In the absence of selection pressure, after 120 h of cultivation, about 50% of cells lost the plasmid. In the presence of selection pressure (kanamycin 25 μg ml − ), after 120 h of cultivation, more than 90% of the cells harboured the plasmid.