Open AccessThe cloning and nucleotide sequence of the serine protease gene ( aspA ) of Aeromonas salmonicida ssp. salmonicida
Author(s) -
Whitby P.W.,
Landon M.,
Coleman G.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05543.x
Subject(s) - subcloning , aeromonas salmonicida , biology , nucleic acid sequence , ribosomal binding site , microbiology and biotechnology , peptide sequence , serine protease , consensus sequence , open reading frame , molecular cloning , gene , genetics , biochemistry , protease , plasmid , ribosome , enzyme , rna , bacteria
The gene for Aeromonas salmonicida serine protease has been cloned into phagemid pTZ18R in two restriction fragments, 2.0‐kb Pst I and 2.3‐kb Kpn I, of genomic DNA. The nucleotide sequences of the two fragments have been determined, in both directions, after subcloning, by double‐stranded sequencing of nested deletions. An open reading frame of 1863 bp translated into a sequence of 621 amino acids, a 24‐amino acid signal peptide and a 597‐amino acid mature enzyme of molecular mass 64 173 Da. The consensus sequence, NGTS, of a serine protease substrate primary binding site was identified and a putative ribosome‐binding site GGAG occurred 6 bp upstream of the ATG initiation codon.