Open AccessCadmium‐resistance plasmid in Staphylococcus lugdunensis
Author(s) -
PoitevinLater Françoise,
Vandenesch François,
Dyke Keith,
Fleurette Jean,
Etienne Jerome
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05542.x
Subject(s) - plasmid , staphylococcus lugdunensis , microbiology and biotechnology , biology , staphylococcus aureus , cadmium , restriction enzyme , genetics , gene , bacteria , staphylococcus , chemistry , organic chemistry
Plasmids that confer resistance to cadmium ( MIC ≧ 125 μ M ), were found in 18 out of 30 independent Staphylococcus lugdunensis strains from clinical specimens. Variants that were cured of their plasmid were cadmium sensitive. Restriction endonuclease sites of a 3.2‐kb cadmium‐resistance plasmid of S. lugdunensis , designated pLUG10, were similar to those of pOX6, a S. aureus cadmium‐resistance plasmid containing the cadB gene. Southern‐blot hybridisation was performed with a probe intragenic to cadB . Hybridisation was found with the cadB probe in the cadmium‐resistant S. lugdunensis isolates to the 2.9‐, 3.2‐ and 3.7‐kb plasmids. These findings suggest that cadmium‐resistance in some S. lugdunensis strains is due to a gene sharing homology with the cadB gene of S. aureus .