Open AccessUltrastructure of the cell wall of the triangular halophilic archaebacterium Haloarcula japonica strain TR‐1
Author(s) -
Nishiyama Yayoi,
Takashina Tomonori,
Grant William D.,
Horikoshi Koki
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05539.x
Subject(s) - ultrastructure , strain (injury) , cell wall , electron microscope , hexagonal crystal system , halophile , biology , biophysics , crystallography , materials science , chemistry , anatomy , bacteria , botany , optics , genetics , physics
Cells of the halophilic archaebacterium Haloarcula japonica strain TR‐1 were prepared for electron microscopy using conventional fixation and the freeze‐substitution method. Cell walls were not observed in thin sections of cells prepared by conventional procedures, whereas thin sections of cells prepared by the freeze‐substitution method revealed a cell wall 20–25 nm in thickness composed of three layers. Arrays of hexagonal structures arranged on the cell surface were observed in grazing sections.