Open AccessNucleotide sequence of the nfa A gene encoding the antigen 8786 adhesive factor of enterotoxigenic Escherichia coli
Author(s) -
Aubel Dominique,
DarfeuilleMichaud Arlette,
Martin Christine,
Joly Bernard
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05527.x
Subject(s) - gene , nucleic acid sequence , biology , genetics , homology (biology) , primer extension , open reading frame , escherichia coli , microbiology and biotechnology , peptide sequence , nucleotide
The nucleotide sequence of a 714‐bp DNA fragment containing the enterotoxic Escherichia coli (ETEC) adhesive factor 8786 structural gene, designated nfa A, revealed an open reading frame of 498 bp encoding a polypeptide of 166 amino acids. Primer‐extension experiments showed that the nfa A gene is within a single transcription unit. No homology was found with the ETEC adhesive factors already sequenced. In contrast, a homology with Salmonella enteritidis fimbrin SEF 14 was observed.