Open AccessSiderophores and related outer membrane proteins produced by pseudomonads isolated from eels and freshwater
Author(s) -
Aznar Rosa,
Alcaide Elena
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05526.x
Subject(s) - siderophore , bacterial outer membrane , microbiology and biotechnology , pseudomonadaceae , biology , bacteria , strain (injury) , enterobactin , pseudomonadales , pseudomonas aeruginosa , salmonella , agar , pseudomonas , chemistry , biochemistry , escherichia coli , gene , genetics , anatomy
A total of 46 environmental pseudomonads, together with six type strains, were examined for their siderophore‐producing activity. All strains were able to grow under iron‐limiting conditions, gave orange halos in the CAS agar assay, and produced hydroxamates, and some of them also produced phenolate‐type compounds. Bioassays showed that all strains, except Pseudomonas aeruginosa , promoted growth of mutant strain Arthrobacter flavescens JG‐9, deficient in hydroxamate production, and some of them promoted growth of Salmonella typhimurium enb‐1, which requires enterobactin for growth. The presence of iron‐regulated outer membrane proteins was observed, the molecular size of the main induced proteins ranged between 76 and 93 kDa.