Open AccessTemperature‐sensitive mutants of the mycobacterium plasmid pAL5000
Author(s) -
Guilhot Christophe,
Gicquel Brigitte,
Martín Carlos
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05510.x
Subject(s) - plasmid , mutant , microbiology and biotechnology , mycobacterium , biology , bacteria , genetics , gene
Two plasmids were isolated as thermosensitive replicons following in vitro mutagenesis of pB4, a pAL5000 derivative mycobacteria/ Escherichia coli shuttle plasmid. Plasmids pCG59 and pCG63 replicate at 30°C but not at 39°C. This will allow their utilisation for transposon delivery, site‐specific integration, or allele exchange.