Open AccessMineralization of 2‐chloro‐ and 2,5‐dichlorobiphenyl by Pseudomonas sp. strain UCR2
Author(s) -
Hickey W.J.,
Brenner V.,
Focht D.D.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05509.x
Subject(s) - strain (injury) , biology , microbiology and biotechnology , pseudomonas , pseudomonas putida , pseudomonadaceae , arthrobacter , pseudomonadales , bacteria , dna–dna hybridization , genomic dna , dna , genetics , anatomy
Pseudomonas sp. strain UCR2 was isolated from a multi‐chemostat mating experiment between a chlorobenzoate‐degrader, Pseudomonas aeruginosa strain JB2, and a chlorobiphenyl‐degrader, Arthrobacter sp. strain B1Barc. Strain UCR2 differed from either of the parental organisms in that it grew on both 2‐chloro‐ and 2,5‐dichlorobiphenyl with concomitant release of chloride. Phenotypic typing by the Biolog TM system indicated that strain UCR2 shared greater similarity with strain JB2 (88%) than strain B1Barc (3%). In DNA: DNA hybridization experiments, genomic DNA from strain UCR2 hybridized with both strain JB2 and strain B1Barc, with the former pairing yielding a much stronger signal than the latter. In contrast, no hybridization whatsoever was observed when the parental organisms strains JB2 and B1Barc were probed against each other.