Open AccessAn FNR‐dependent promoter from Escherichia coli is active and anaerobically inducible in Paracoccus denitrificans
Author(s) -
Spiro Stephen
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05504.x
Subject(s) - paracoccus denitrificans , escherichia coli , biology , plasmid , regulator , gene , transcriptional regulation , promoter , microbiology and biotechnology , bacteria , gene expression , biochemistry , genetics
A transcriptional fusion of a synthetic FNR‐dependent promoter derived from Escherichia coli has been introduced into Paracoccus denitrificans on a broad‐host‐range plasmid. The patterns of expression of β‐galactosidase from this fusion and from a control which is not regulated by FNR have been studied. The results indicate that P. denitrificans expresses a transcriptional regulator which has a very similar DNA‐binding specificity to FNR, and responds to a similar physiological signal.