Open AccessDetection and differentiation of Colletotrichum gloeosporioides isolates using PCR
Author(s) -
Mills Peter R.,
Sreenivasaprasad S.,
Brown Averil E.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05503.x
Subject(s) - rapd , primer (cosmetics) , ribosomal dna , biology , polymerase chain reaction , microbiology and biotechnology , dna , colletotrichum gloeosporioides , internal transcribed spacer , ribosomal rna , spacer dna , oligonucleotide , genetics , gene , botany , genetic diversity , phylogenetics , chemistry , population , demography , organic chemistry , sociology
An oligonucleotide primer (CgInt), synthesised from the variable internally transcribed spacer (ITS) 1 region of ribosomal DNA (rDNA) of Colletotrichum gloeosporioides was used for PCR with primer ITS4 (from a conserved sequence of the rDNA) to amplify a 450‐bp fragment from the 25 C. gloeosporioides isolates tested. This specific fragment was amplified from as little as 10 fg of fungal DNA. A similar sized fragment was amplified from DNA extracted from C. gloeosporioides ‐infected tomato tissue. RAPD analysis divided 39 C. gloeosporioides isolates into more than 12 groups linked to host source and geographic origin. Based on the results obtained, the potential of PCR for detection and differentiation of C. gloeosporioides is discussed.