Open AccessCloning and expression in Escherichia coli of a phoA gene encoding a phosphate‐irrepressible alkaline phosphatase of Zymomonas mobilis
Author(s) -
Michel Gérard P.F.,
Alvarez Elisa,
Guzzo Jean,
Cami Brigitte,
Baratti Jacques
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05497.x
Subject(s) - zymomonas mobilis , mutant , alkaline phosphatase , cloning (programming) , gene , escherichia coli , biology , microbiology and biotechnology , phosphatase , biochemistry , enzyme , ethanol , computer science , programming language , ethanol fuel
The Zymomonas mobilis phoA gene, encoding a phosphate‐irrepressible alkaline phosphatase (ZAPase), was cloned and its expression was studied in phoA mutants of Escherichia coli . The ZAPase was recovered in the soluble fraction of E. coli . The enzyme was synthesized constitutively and its synthesis not repressed by phosphate, unlike the phoA gene of E. coli . The phoA gene of Z. mobilis was mutagenized by Mini Mu PR13 and the mutated gene crossed into Z. mobilis in order to obtain phoA mutants by reverse genetics. Although Z. mobilis mutants with Mini Mu PR13 integrated in the chromosome were obtained, none had an allele replacement for none was defective in ZAPase.