Open AccessStructure of mycoside F, a family of trehalose‐containing glycolipids of Mycobacterium fortuitum
Author(s) -
Gautier Nicolas,
Marín Luz María López,
Lanéelle MarieAntoinette,
Daffé Mamadou
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05494.x
Subject(s) - glycolipid , mycobacterium fortuitum , trehalose , mass spectrometry , chemistry , glycoconjugate , biovar , nuclear magnetic resonance spectroscopy , biochemistry , chromatography , mycobacterium , bacteria , stereochemistry , biology , gene , genetics
Nuclear magnetic resonance spectroscopy, fast‐atom bombardment mass spectrometry, gas chromatography‐mass spectrometry, as well as chemical degradations were used to elucidate the structure of the major glycolipids of Mycobacterium fortuitum . Three main glycoconjugates were detected and their structures established as 2,3‐diacyl, 2,3,4‐ and 2,3,6‐triacyl trehalose. The characteristic infrared spectrum which led to their original designation as mycoside F, a family of glycolipids limited in distribution M. fortuitum , was due to the nature of the fatty acyl substituents identified primarily as 2‐methyl‐octadecen‐2‐oyl. The antigenic glycolipids typified the biovar. fortuitum , thus allowing its easy recognition from the C‐mycoside glycopeptidolipid‐containing biovar. peregrinum .