Open AccessActivation of neutral trehalase by fermentable sugars and cAMP in the fission yeast Schizosaccharomyces pombe
Author(s) -
Carrillo Dolores,
VicenteSoler Jeronima,
Gacto Mariano
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05490.x
Subject(s) - trehalase , schizosaccharomyces pombe , cycloheximide , biochemistry , adenylate kinase , cyclase , yeast , enzyme , chemistry , stimulation , biology , saccharomyces cerevisiae , protein biosynthesis , endocrinology
Derepressed cells of Schizosaccharomyces pombe 972 h − suspended in the presence of glucose or other fermentable sugars displayed a transient activation of trehalase which was not blocked by cycloheximide. Repressed cells were unable to show glucose‐induced trehalase stimulation. Nitrogen sources, protonophores or uncouplers failed to produce direct trehalase activation but increased the activity of the enzyme in the presence of glucose. Exogenous cAMP induced a rapid and pronounced stimulation of trehalase in both repressed and derepressed cells suggesting that the response to glucose includes activation of adenylate cyclase as part of a cAMP signalling pathway that increases the catalytic activity of trehalase by enzyme modification.