Open AccessHigh‐frequency deletion involving closely spaced rRNA gene sets in Streptococcus thermophilus
Author(s) -
Pébay Mireille,
Roussel Yvonne,
Simonet JeanMarc,
Decaris Bernard
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05488.x
Subject(s) - genetics , biology , locus (genetics) , homologous chromosome , homologous recombination , gene , mutant , ribosomal rna , recombination , streptococcus thermophilus , microbiology and biotechnology , bacteria , lactobacillus
Homologous rDNA probes were used to study the number of rRNA genes in a five‐generation genealogy of Streptococcus thermophilus CNRZ368. Whereas the CNRZ368 strain contained six rRNA loci, four independant mutants with five rrn loci were obtained. The deletion frequency was 5 × 10 −2 . Molecular analysis provided identical hybridization patterns for all the deletion mutants. The deletion was shown to occur within the two close rRNA loci, rrnD and rrnE , probably due to a homologous recombination event and to give rise to a hybrid rrnD/E locus.