Open AccessConjugative trimethoprim resistance in Staphylococcus aureus
Author(s) -
Udo E.E.,
Wei M.Q.,
Grubb W.B.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05470.x
Subject(s) - plasmid , ecori , kanamycin , staphylococcus aureus , microbiology and biotechnology , biology , restriction enzyme , genetics , dna , bacteria , antibiotics
A multiply resistant Staphylococcus aureus isolate, WBG7410, harbours plasmids of 38, 26, 2.8, 2.4 and 1.9 kb and transfers trimethoprim and kanamycin resistance at high frequencies by conjugation. The transconjugants contained the 38‐kb plasmid, pWBG707, and the 2.8‐kb plasmid. Plasmid pWBG707 was shown to encode trimethoprim resistance, was conjugative and mobilised at high frequencies the 2.8‐kb plasmid which presumably encodes kanamycin resistance. Plasmid pWBG707 was isolated mostly in the open circular form and analysis with Eco RI restriction endonuclease suggests that pWBG707 is a new conjugative plasmid distinct from the other conjugative plasmids reported in S. aureus .