Open AccessTCP pilus biosynthesis in Vibrio cholera O1: gene sequence of tcpC encoding an outer membrane lipoprotein
Author(s) -
Ogierman Monica A.,
Manning Paul A.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05459.x
Subject(s) - operon , bacterial outer membrane , gene , signal peptidase , signal peptide , nucleic acid sequence , vibrio cholerae , biology , sequence (biology) , escherichia coli , peptide sequence , biochemistry , genetics , chemistry , bacteria
The nucleotide sequence of the tcpC gene has been determined. It encodes a 53995‐Da protein precursor with a signal sequence and cleavage site typical of a number of outer membrane lipoproteins, which are cleaved by the equivalent of signal peptidase II (Lsp) of Escherichia coli . The location of the tcpC gene is such that it is predicted to be translationally coupled to the 5′ and 3′ flanking genes, tcpY and tcpD , respectively, indicating that it forms part of an operon. Together with the lipoprotein signal sequence and the several hydrophobic domains it seems likely that TcpC is a surface‐anchored trans‐outer membrane lipoprotein.