Open AccessATP synthesis in Halobacterium saccharovorum : Evidence that synthesis may be catalysed by an F 0 F 1 ‐ATP synthase
Author(s) -
Hochstein Lawrence I.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05455.x
Subject(s) - atp synthase , atpase , atp hydrolysis , enzyme , v atpase , biochemistry , chemistry , chemiosmosis , cyanide , adenosine triphosphate , stereochemistry , biology , organic chemistry
Halobacterium saccharovorum synthesized ATP in response to a pH shift from 8 to 6.2. Synthesis was inhibited by carbonyl cyanide m ‐chloro‐phenylhydrazone, dicyclohexylcarbodiimide, and azide. Nitrate, an inhibitor of the membrane‐bound ATPase previously isolated from this organism, did not inhibit ATP synthesis. N ‐Ethylmaleimide, which also inhibited this ATPase, stimulated the production of ATP. These observations suggested that H. saccharovorum synthesized and hydrolysed ATP using different enzymes and that the vacuolar‐like ATPase activity previously described in H. saccharovorum was an ATPase whose function is yet to be identified.