Open AccessCloning and sequencing of a plasmid‐mediated erythromycin resistance determinant from Staphylococcus xylosus
Author(s) -
Milton Ian D.,
Hewitt Carey L.,
Harwood Colin R.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05453.x
Subject(s) - plasmid , msra , biology , staphylococcus xylosus , staphylococcus epidermidis , genetics , open reading frame , bacillus subtilis , microbiology and biotechnology , gene , molecular cloning , cloning (programming) , nucleic acid sequence , staphylococcus , staphylococcus aureus , peptide sequence , bacteria , amino acid , methionine , computer science , programming language
A 2.3‐kb DNA fragment cloned from plasmid pCH200, the largest (52 kb) of four plasmids detected in Staphylococcus xylosus , was found to confer resistance to 14‐membered ring macrolides in Bacillus subtilis and Staphylococcus aureus . DNA‐sequence analysis of the fragment revealed the presence of an open‐reading frame, the deduced product of which was identical to one of the two ATP‐binding domains encoded by the macrolide/streptogramin‐B‐resistance gene msrA of Staphylococcus epidermidis . The observation that a polypeptide homologous to the C‐terminus of MsrA is capable of mediating erythromycin resistance in the absence of the N‐terminal region is of significance both to the evolution and functional activity of members of the ATP‐binding transport super‐gene family.