Open AccessComparison of the nucleotide sequence and development of a PCR test for the epsilon toxin gene of Clostridium perfringens type B and type D
Author(s) -
Harvard Helen L.,
Hunter Sophie E.C.,
Titball Richard W.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05443.x
Subject(s) - clostridium perfringens , nucleic acid sequence , gene , open reading frame , biology , toxin , peptide sequence , microbiology and biotechnology , clostridiaceae , sequence (biology) , polymerase chain reaction , genetics , nucleotide , sequence analysis , bacteria
The sequence of the epsilon toxin gene of Clostridium perfringens type D was determined and compared with that of the previously reported type B sequence. It showed two nucleotide changes in the open reading frame, giving rise to one amino acid substitution. The promoter sequences were not homologous, and different putative −35 and −10 regions have been identified in each. The sequence information was used to develop PCR primers which were specific for the epsilon toxin gene. The utility of this system for identifying type B or D strains of C. perfringens was demonstrated.