Open AccessGentisate pathway in Salmonella typhimurium : metabolism of m ‐hydroxybenzoate and gentisate
Author(s) -
Goetz Frederick E.,
Harmuth Lorna J.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05437.x
Subject(s) - hydroxybenzoate , bacteria , biochemistry , enterobacteriaceae , enzyme , chemistry , salmonella , isomerase , biology , escherichia coli , genetics , gene
Salmonella typhimurium was shown to use the gentisate pathway to metabolize m ‐hydroxybenzoate and gentisate. m ‐Hydroxybenzoate hydroxylase and gentisate 1,2‐dioxygenase were induced by growth on either gentisate or m ‐hydroxybenzoate. These enzymes were not detected when the bacteria were grown with glucose or glucose and either m ‐hydroxybenzoate or gentisate. However, both enzymes were induced when the bacteria were grown on succinate with either substrate. The maleylpyruvate isomerase required reduced glutathione and was irreversibly inhibited by N ‐ethylmaleimide.