Open AccessIn vivo recombination and the production of hybrid genes
Author(s) -
Calogero Sabina,
Bianchi Marco E.,
Galizzi Alessandro
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05436.x
Subject(s) - plasmid , homologous recombination , gene , biology , genetics , cloning (programming) , recombination , escherichia coli , flp frt recombination , dna , molecular cloning , function (biology) , homologous chromosome , in vitro recombination , genetic recombination , gene expression , computer science , programming language
In vivo recombination between homologous genes is increasingly being favoured as a means of generating proteins with altered and novel specificities. The typical procedure requires the cloning of two related genes on a single replicative plasmid of Escherichia coli and the selection or screening of recombinants. Up to now the recombination process between the cloned genes was generally thought to involve the recA function and the availability of free ends in the DNA molecule to be recombined. Our results show that neither is necessary. Recombinants are obtained by simply growing the bacteria that host the plasmid carrying the two cloned genes.