Open AccessIdentification of 27‐oxo‐octacosanoic acid and heptacosane‐1,27‐dioic acid in Legionella pneumophila
Author(s) -
Moll Hermann,
Sonesson Anders,
Jantzen Erik,
Marre Reinhard,
Zähringer Ulrich
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05430.x
Subject(s) - chloroform , chemistry , phenol , fatty acid , gas chromatography , chromatography , organic chemistry , ether , legionella pneumophila , petroleum ether , mass spectrometry , bacteria , extraction (chemistry) , biology , genetics
Two long‐chain fatty acids, 27‐oxo‐octacosanoic acid (28:0(27‐oxo)) and heptacosane‐1,27‐dioic acid (27:0‐dioic) were identified for the first time in phenol‐chloroform‐petroleum ether extracts of Legionella pneumophila , indicating that they are constituents of lipopolysaccharide. The fatty acids were characterised by combined gas‐liquid chromatography/mass spectrometry and proton nuclear magnetic resonance spectroscopy. Moreover, minor amounts of 29‐oxo‐triacontanoic (30:0(29‐oxo)) acid and nonacosane‐1,29‐dioic acid (29:0‐dioic) as well as 27‐hydroxy‐octacosanoic acid (28:0(27‐OH)) were present in the phenol‐chloroform‐petroleum ether extract.