Open AccessThe mechanism of inhibition by EDTA and EGTA of methanol oxidation by methylotrophic bacteria
Author(s) -
Chan H.T.C.,
Anthony C.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05422.x
Subject(s) - egta , bacteria , chemistry , methanol , mechanism (biology) , biochemistry , biophysics , chromatography , biology , calcium , organic chemistry , philosophy , genetics , epistemology
Ethyleneglycol (aminoethylether) tetra‐acetic acid (EGTA) was shown to be a potent competitive inhibitor of electron transfer between methanol dehydrogenase (MDH) and its electron acceptor cytochrome c L . Addition of Ca 2+ ions relieved the inhibition by removal of the inhibitory EGTA. Removal of EGTA by gel filtration completely relieved the inhibition. EGTA did not remove the tightly bound Ca 2+ present in the MDH. Indo‐1, a fluorescent analogue of EGTA, bound tightly to MDH in a 1 : 1 ratio but not to cytochrome c L ; binding was prevented by EGTA. It was concluded that EGTA inhibits methanol oxidation by binding to lysyl or arginyl residues on MDH thus preventing docking with cytochrome c L .