Open AccessOccurrence of cold‐labile NAD‐specific glutamate dehydrogenase in Bacillus species
Author(s) -
Jahns Thomas
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05414.x
Subject(s) - nad+ kinase , nicotinamide adenine dinucleotide , biochemistry , cereus , dehydrogenase , biology , glutamate dehydrogenase , bacillales , enzyme , bacillus sphaericus , microbiology and biotechnology , incubation , bacillus cereus , chemistry , bacillus subtilis , bacteria , glutamate receptor , genetics , receptor
A nicotinamide adenine dinucleotide‐specific glutamate dehydrogenase (NAD‐GluDH; EC 1.4.1.3) inactivated by incubation at low temperatures was detected in several species of the genus Bacillus , including strains of B. cereus, B. laterosporus, B. lentus, B. panthotenicus, B. pasteurii, B. sphaericus, B. stearothermophilus, B. subtilis and B. thuringiensis . Incubation of cell‐free extracts of these strains at 0°C resulted in an 80–100% inactivation of NAD‐GluDH activity within 120 min. The addition of 20% glucerol protected the enzyme from this inactivation in the cold. Strains of B. fastidiosus, B. licheniformis, B. macerans , B. megaterium and B. pumilus were found to lack NAD‐GluDH activity.