Open AccessCloning and characterization of an amplified DNA sequence in chromosomal DNA of Streptomyces aureofaciens 2201
Author(s) -
Pristaš Peter,
Godány Andrej
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05411.x
Subject(s) - extrachromosomal dna , replicon , biology , genetics , dna , strain (injury) , cloning (programming) , microbiology and biotechnology , dna sequencing , chromosome , gene , plasmid , computer science , programming language , anatomy
In strain 2201 of Streptomyces aureofaciens , a high copy number amplified DNA sequence (ADS‐Sa2201) was found and characterized. The amplified sequence in the chromosomal DNA of this strain forms a strecth of about 10 kb tandemly repeated 100–500 times. In this strain also, extrachromosomal copies of the repeated unit of the ADS‐Sa2201 were found. In cloning experiments any autonomous replicon was found on ADS‐Sa2201 and it thus can be presumed that the presence of the extrachromosomal copies of the repeated unit of ADS‐Sa2201 is only a result of its excision from the chromosome. A part of the repeated unit of ADS‐Sa2201 was also found in chromosomal DNA of strain 13 of S. aureofaciens . In this strain no part of ADS‐Sa2201 is present extrachromosomally.