Open AccessExtraction and characterization of lipopolysaccharide from Pseudomonas pseudomallei
Author(s) -
Kawahara Kazuyoshi,
Dejsirilert Surang,
Danbara Hirofumi,
Ezaki Takayuki
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05405.x
Subject(s) - pseudomonas , microbiology and biotechnology , lipopolysaccharide , extraction (chemistry) , pseudomonadales , pseudomonadaceae , chemistry , bacteria , biology , chromatography , immunology , genetics
The best yield of lipopolysaccharide (LPS) of Pseudomonas pseudomallei GIFU 12046 was obtained by extraction of defatted cells by phenol/chloroform/petroleum ether. The LPS showed a smooth character on SDS‐polyacrylamide gel electrophoresis and contained d ‐glucose, l ‐ glycero ‐ d ‐ manno ‐heptose, and d ‐glucosamine as the main sugar components, and 3‐hydroxypalmitic acid as an amide‐linked fatty acid. The growth conditions did not affect the electrophoresis profile and chemical composition of LPS. 2‐ Keto ‐3‐deoxyoctonic acid was not detectable, and mild acid hydrolysis could not liberate free lipid A, suggesting that the linkage between inner core and lipid A was stable against acid hydrolysis, and the structure of this region is similar to that of P. cepacia , which has close taxonomic relationship with P. pseudomallei .