Open AccessEsterase electrophoresis compared with biotyping for epidemiological typing of Acinetobacter baumannii strains
Author(s) -
Pouëdras P.,
Gras S.,
Sire J.M.,
Mesnard R.,
Donnio P.Y.,
Picard B.,
Avril J.L.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05404.x
Subject(s) - typing , esterase , acinetobacter baumannii , microbiology and biotechnology , neisseriaceae , biology , acinetobacter , gel electrophoresis , electrophoresis , enzyme , bacteria , genetics , biochemistry , antibiotics , pseudomonas aeruginosa
Forty‐nine Acinetobacter baumannii strains belonging to three biotypes and isolated from four hospitals were differentiated by electrophoretic typing of their esterases. Six main kinds of esterases were distinguished by their spectra of hydrolytic activity toward seven synthetic substrates. The electrophoretic variations of these enzymes were used to define ten zymotypes among the three biotypes. Esterase electrophoresis appeared to be more sensitive than biotyping, and could represent an additional marker for epidemiological analysis.