Open AccessThe serotyping of Azospirillum spp. by cell‐gold immunoblotting
Author(s) -
Bogatyrev Vladimir A.,
Dykman Lev A.,
Matora Larisa Yu.,
Schwartsburd Boris I.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05402.x
Subject(s) - serotype , microbiology and biotechnology , biology
Ten Azospirillum strains were serotyped using the method of cell‐gold immunoblotting (dot‐blot immune‐overlay assay). Colloidal gold‐protein A conjugate was used. Antibodies raised against the whole cells showed strain specificity and interacted mainly with carbohydrate antigens on the cell surface. Immunological identity for A. brasilense Sp 245 and Sp 107 strains was found. Cell‐gold immunoblotting can be recommended for serotyping of a wide variety of bacterial strains.