Open AccessStably inherited killer activity in industrial yeast strains obtained by electrotransformation
Author(s) -
Salek A.,
Schnettler R.,
Zimmermann U.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05400.x
Subject(s) - mutant , yeast , saccharomyces cerevisiae , strain (injury) , microbiology and biotechnology , biology , saccharomyces , industrial microbiology , gene , fermentation , biochemistry , anatomy
Killer‐sensitive strains of Saccharomyces cerevisiae and Saccharomyces carlsbergensis were transformed by electroinjection using double‐stranded RNA isolated from a superkiller strain. Various recipient strains were used: both thermo‐resistant and thermo‐sensitive as well as mutants of industrial strains. Conversion of respiratory competent ( rho + ) into respiratory deficient ( rho − ) strains (mutants) resulted in a significant increase of the yield of electrotransformants and/or of longterm killer stability. Electrotransformation of rho − mutants of distillery and brewery strains resulted in more than 100 clones, which exhibited weak or strong killer activity over some or all of the experimental period of 10 months.