Open AccessDegradation of chlorotoluenes by in vivo constructed hybrid strains: problems of enzyme specificity, induction and prevention of meta ‐pathway
Author(s) -
Brinkmann Ulrich,
Reineke Walter
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05397.x
Subject(s) - pseudomonas putida , strain (injury) , pseudomonas , enzyme , microbiology and biotechnology , dehydrogenase , chemistry , biochemistry , plasmid , degradation (telecommunications) , biology , bacteria , gene , genetics , anatomy , telecommunications , computer science
The activities of the TOL plasmid‐coded xylene oxygenase, benzylalcohol dehydrogenase, benzaldehyde dehydrogenase of Pseudomas putida strain PaW1 were tested with substituted toluenes, benzylalcohols and benzaldehydes, respectively, as substrates. Several chlorinated toluenes were shown to induce enzymes of the xylene degradation sequence. Conjugative transfer of the TOL plasmid from Pseudomonas putida strain PaW1 to Pseudomonas sp. strain B13 and Pseudomonas cepacia strain JH230 allowed the isolation of hybrid strains capable of growing in the presence of 3‐chloro, 4‐chloro‐ and 3,5‐dichlorotoluene. Hybrid strains revealed new ways to prevent the dead‐end meta ‐pathway for chlorocatechols.