Open AccessRestriction fragment length polymorphisms in mitochondrial DNA and ribosomal RNA gene complexes as an aid to the characterization of species and sub‐species populations in the genus Verticillium
Author(s) -
Typas Milton A.,
Griffen Alison M.,
Bainbridge Brian W.,
Heale James B.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05359.x
Subject(s) - haeiii , biology , ecori , restriction fragment length polymorphism , restriction enzyme , genetics , mitochondrial dna , ribosomal rna , genomic dna , microbiology and biotechnology , restriction fragment , verticillium , gene , polymerase chain reaction , botany
Genomic DNA was extracted from seven species of Verticillium and digested with the restriction endonucleases Eco RI or Hae III. Hybridization with an homologous V. albo‐atrum ribosomal RNA gene probe revealed restriction fragment length polymorphisms (RFLPs) which could differentiate V. lateritium, V. lecanii, V. nigrescens, V. nubilum and V. tricorpus . Digestion with Eco RI did not provide RFLPs which could distinguish between V. albo‐atrum and V. dahliae . Digestion of genomic and mitochondrial DNA with Hae III showed distinctive patterns on ethidium bromide gels which allowed each species to be distinguished. Some intra‐species variation in patterns occurred and a combination of mitochondrial and ribosomal RNA gene complex RFLPs has potential as an aid for the characterization of species and sub‐species populations in the genes Verticillium .