Open AccessPeroxisome biogenesis in Hansenula polymorpha : different mutations in genes, essential for peroxisome biogenesis, cause different peroxisomal mutant phenotypes
Author(s) -
Titorenko Vladimir I.,
Waterham Hans R.,
Haima Peter,
Harder Wim,
Veenhuis Marten
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05357.x
Subject(s) - mutant , peroxisome , biology , phenotype , biogenesis , gene , genetics , mutation , microbiology and biotechnology
In Hansenula polymorpha , different monogenic recessive mutations mapped in either of two previously identified genes, PER1 and PER3 , produced different peroxisomal mutant phenotypes. Among five per1 mutants, four showed a Pim − phenotype: the cells contained few small peroxisomes while the bulk of the matrix enzymes resided in the cytosol. One of these mutants, per1–124 had an enhanced rate of peroxisome proliferation. The fifth mutant completely lacked peroxisomes (Per − phenotype). Of seven per3 mutants, four displayed a Pim − phenotype, two others a Per − phenotype, while one mutant showed pH‐dependent growth on methanol and was affected in oligomerization or peroxisomol matrix protein. Thus, the protein products of both PER1 and PER3 genes appear to be essential in different aspects of peroxisome assembly/ proliferation.