Open AccessNitrosating activity in Escherichia coli
Author(s) -
O'Donnell Claire M.,
Edwards Clive
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05348.x
Subject(s) - nitrosation , nitrite reductase , nitrite , escherichia coli , mutant , biochemistry , chemistry , reductase , enzyme , nitrate reductase , organic chemistry , nitrate , gene
Nitrosation activity was measured in Escherichia coli isolates and a range of nitrite reductase ( nir ) mutants. Activity was only detected in intact cells and could be inhibited by a number of treatments such as sonication and osmotic shock. Aerobically‐grown cells had highest nitrosation activity compared to oxygen‐limited ones. Inclusion of nitrite in growth media induced high activities of nitrite reductase and for some isolates, nitrosation. Analysis of nir mutants identified two which were unable to nitrosate. This result suggested that NADH‐dependent nitrite reductase was implicated either directly or indirectly in nitrosation.