Open AccessAnalysis of DNA encoding 23S rRNA and 16S–23S rRNA intergenic spacer regions from Plesiomonas shigelloides
Author(s) -
East Alison K.,
Allaway D.,
Collins M.D.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05342.x
Subject(s) - 23s ribosomal rna , ribosomal rna , biology , spacer dna , genetics , internal transcribed spacer , intergenic region , nucleic acid sequence , plesiomonas shigelloides , ribosomal intergenic spacer analysis , gene , 5s ribosomal rna , microbiology and biotechnology , rna , 18s ribosomal rna , genome , bacteria , ribosome
Amplification of the gene encoding 23S rRNA of Plesiomonas shigelloides by polymerase chain reaction (PCR), with primers complementary to conserved regions of 16S and the 3′ end of 23S rRNA genes, resulted in a DNA fragment of approximately 3 kb. This fragment was cloned in Escherichia coli and its nucleotide sequence determined. The region encoding 23S rRNA shows high homology with the published sequences of 23S rRNA from other members of the gamma division of Proteobacteria . The sequence of the intergenic spacer region, between the 16S and 23S rRNA genes, was determined in a further two clones. In one the sequence of a single tRNA Glu was found which was absent from the other two. This variation in sequence suggests that the different clones may be derived from different ribosomal RNA operons.