Open AccessMycobacterium avium and Mycobacterium intracellulare chromatotypes defined by curvilinear gradient HPLC of mycolic acids
Author(s) -
Duffey Paul S.,
Guthertz Linda S.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05338.x
Subject(s) - mycobacterium , high performance liquid chromatography , bacteria , mycolic acid , microbiology and biotechnology , biology , chromatography , mycobacterium avium complex , chemistry , genetics
Seventy‐nine strains of Mycobacterium avium complex bacteria (MAC), previously characterized by genetic probe analysis, were assayed using two methods of reverse phase high‐performance liquid chromatography (HPLC) that employed curvilinear gradients. Although different in column length and cycle time, the methods produced equivalent results, yielding seven distinct chromatographic patterns (chromatotypes) of M. avium and M. intracellulare based on the ratio of mycolate concentrations in the late vs. the middle of three peak clusters (L:M ratio). The M. avium strains ( n = 36) were assigned to chromototypes I through 4 (L:M ratios < 3), and the M. intracellulare strains ( n = 24) to chromototypes 5 through 7 (L:M ratios > 4). Of 18 Mycobacterium ‘X’ strains, seven resembled M. avium , seven others resembled M. intracellular , and four were intermediate between M. avium and M. intracellulare .