Open AccessInsertion into the Mycobacterium smegmatis genome of the aph gene through lysogenization with the temperate mycobacteriophage Ms6
Author(s) -
Anes Elsa,
Portugal Isabel,
MonizPereira J.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05337.x
Subject(s) - mycobacterium smegmatis , biology , gene , transposable element , genome , genetics , temperateness , microbiology and biotechnology , bacteriophage , mycobacterium tuberculosis , escherichia coli , medicine , tuberculosis , pathology
A derivative of the temperate mycobacteriophage Ms6 containing the aph gene from transposon Tn 5 was constructed. In the transductants the aph gene was integrated in the bacterial genome. The aph gene is stably maintained in the absence of positive selection after more than 150 generations. The results presented in this report show that Ms6 can be used as a vehicle for the integration of foreign DNA into the Mycobacterium smegmatis genome. a