Open AccessCloning of a Bacteroides fragilis chromosomal determinant coding for 5‐nitroimidazole resistance
Author(s) -
Haggoud A.,
Reysset G.,
Sebald M.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05334.x
Subject(s) - plasmid , insert (composites) , bacteroides fragilis , cloning vector , biology , cloning (programming) , molecular cloning , nitroimidazole , genomic dna , recombinant dna , southern blot , microbiology and biotechnology , dna , chemistry , genetics , vector (molecular biology) , gene , antibiotics , gene expression , organic chemistry , computer science , programming language , mechanical engineering , engineering
Strain BF8 is a plasmid‐free Bacteroides fragilis , resistant to 5‐nitroimidazole (5‐Ni) antibiotics (metronidazole, ornidazole and tinidazole). The resistance was transferable by conjugation into Bacteroides fragiles BF638R. The total DNA of a Ni r transconjugant was used for the construction of a Sau 3A genomic library in a B. fragilis cloning vector pFK707ΔH1 (4.2 kb). By electrotransformation of strain BF638R, a recombinant plasmid containing an insert of 5.4 kb was obtained which conferred to the host strain the resistance to 5‐Ni. The physical map of the insert was established. After deletion analysis of the insert, the Ni r determinant was localized on a Hpa II‐ Hinc II fragment of 1.6 kb in size. This Ni r determinant has been compared by Southern‐blot analysis with other Bacteroides Ni r determinants of plasmid origin.