Open AccessImmunocharacterization of actin and its immunofluorescent localization during the developmental gametophytic stages of Allomyces arbuscula
Author(s) -
Nguyen Thi BichNga,
Turian Gilbert
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05325.x
Subject(s) - neurospora crassa , polyclonal antibodies , actin , biology , immunofluorescence , actina , microbiology and biotechnology , antibody , biochemistry , cytoskeleton , genetics , gene , cell , mutant
Highly specific polyclonal antibodies against actin from Allomyces arbuscula were produced in rabbits, immunopurified by immunoblotting and specified with actin isolated from Neurospora crassa and mouse skeletal muscle. Used as immunofluorescence probes, they allowed localization of actin in the sequential gametophytic stages of the mould.