Open AccessPlasmid transformation of Ruminococcus albus by means of high‐voltage electroporation
Author(s) -
Cocconcelli Pier Sandro,
Ferrari Elena,
Rossi Filippo,
Bottazzi Vittorio
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05319.x
Subject(s) - electroporation , plasmid , transformation (genetics) , biology , recombinant dna , bacteria , microbiology and biotechnology , transformation efficiency , dna , genetics , gene , agrobacterium
To apply recombinant DNA techniques to the genetic manipulation of cellulolytic ruminal bacteria, a plasmid vector transformation system must be available. The objective of this work was to develop a system for plasmid transformation of Ruminococcus albus . Using high voltage electrotransformation, pSC22 and pCK17 plasmid vectors, derived from lactic acid bacteria plasmids and replicating via single‐stranded DNA intermediate, were successfully introduced into three freshly isolated R. albus strains and into R. albus type strain ATCC 27210. The optimization of the electrotransformation condition raised the electroporation efficiency up to 3 × 10 5 transformants per μg of pSC22 plasmid.