Open AccessIsolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus
Author(s) -
Yamamoto Shigeo,
Fujita Yasuhiro,
Okujo Noriyuki,
Minami Chiaki,
Matsuura Shyoko,
Shinoda Sumio
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05311.x
Subject(s) - vibrio parahaemolyticus , siderophore , ethanolamine , chemistry , alanine , vibrio , mass spectrum , citric acid , strain (injury) , stereochemistry , microbiology and biotechnology , bacteria , chromatography , ion , biochemistry , biology , organic chemistry , amino acid , genetics , anatomy , gene
A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dower 1 × 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 μM added FeCl 3 . Hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2‐ketoglutaric acid. The 1 H‐NMR spectrum exhibited the presence of a residue from each of these components in the intact molecule. The fast‐atom bombardment mass spectrum of the methyl ester derivative indicated a prominent ion at m/z 477, probably corresponding to [M + 1] ion. Other strains of V. parahaemolyticus were also found to produce this compound when grown in an iron‐limited medium.