Open AccessComparative analysis of 23S ribosomal RNA gene sequences of Bacillus anthracis and emetic Bacillus cereus determined by PCR‐direct sequencing
Author(s) -
Ash Carol,
Collins Matthew D.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05292.x
Subject(s) - bacillus anthracis , bacillus cereus , 23s ribosomal rna , ribosomal rna , biology , gene , cereus , rna , microbiology and biotechnology , nucleic acid sequence , genetics , sequence analysis , ribosome , bacteria
The primary structures of the 23S ribosomal RNA genes of Bacillus anthracis and an emetic strain of Bacillus cereus were determined by direct sequencing of enzymatically amplified chromosomal DNA. The 23S rRNA gene sequences of B. anthracis and B. cereus were found to be almost identical and showed only two differences (a single nucleotide change, and a single base insertion in B. cereus ). The feasibility of using PCR‐direct sequencing for the rapid sequence determination of large‐subunit rRNA genes is demonstrated.