Open AccessThymine transport in Lactobacillus casei
Author(s) -
Avraham Y.,
Grossowicz N.,
DeGroot N.,
Yashphe J.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05286.x
Subject(s) - thymine , lactobacillus casei , chemistry , sodium azide , uracil , thymidine , guanosine , biochemistry , dithiothreitol , hypoxanthine , enzyme , fermentation , dna
A transport system for thymine was investigated in a Lactobacillus casei mutant lacking thymidine phosphorylase activity (the first enzyme required for thymine utilization). Transport was dependent on an energy supply; transport was inhibited by the uncouplers carbonylcyanide‐m‐chlorophenylhydrazone (CCCP) and 2,4 dinitrophenol (DNP), and also by sodium azide and N,N′‐dicyclohexylcarbodiimide (DCCD). Thymine transport was inhibited by some uracil and adenosine derivatives and by thymidine, but was not affected by guanosine, deoxycytidine or azacytidine. Inhibition by p‐chloromercurybenzoate (PCMB) was reversed by dithiothreitol (DTT).